Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
Stellar systems composed of single, double, triple or higher-order systems are rightfully regarded as the fundamental building blocks of the Milky Way. Binary stars play an important role in formation and evolution of the Galaxy. Through comparing the radial velocity variations from multiepoch observations, we analyze the binary fraction of dwarf stars observed with LAMOST. Effects of different model assumptions, such as orbital period distributions on the estimate of binary fractions,are investigated. The results based on log-normal distribution of orbital periods reproduce the previous complete analyses better than the power-law distribution. We find that the binary fraction increases with Teff and decreases with [Fe/H]. We first investigate the relation between α-elements and binary fraction in such a large sample as provided by LAMOST. The old stars with high [α/Fe] dominate with a higher binary fraction than young stars with low [α/Fe]. At the same mass, earlier forming stars possess a higher binary fraction than newly forming ones, which may be related with evolution of the Galaxy. 相似文献
The Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST) is the largest existing spectroscopic survey telescope, having 32 scientific charge-coupled-device(CCD) cameras for acquiring spectra. Stability and automation of the camera-control software are essential, but cannot be provided by the existing system. The Remote Telescope System 2 nd Version(RTS2) is an open-source and automatic observatory-control system. However, all previous RTS2 applications were developed for small telescopes. This paper focuses on implementation of an RTS2-based camera-control system for the 32 CCDs of LAMOST. A virtual camera module inherited from the RTS2 camera module is built as a device component working on the RTS2 framework. To improve the controllability and robustness, a virtualized layer is designed using the master-slave software paradigm, and the virtual camera module is mapped to the 32 real cameras of LAMOST. The new system is deployed in the actual environment and experimentally tested. Finally, multiple observations are conducted using this new RTS2-frameworkbased control system. The new camera-control system is found to satisfy the requirements for automatic camera control in LAMOST. This is the first time that RTS2 has been applied to a large telescope, and provides a referential solution for full RTS2 introduction to the LAMOST observatory control system. 相似文献
In many countries, coastal planners strive to balance the demands between civil, commercial strategy and environmental conversation interests for future development, particularly given the sea level rise in the 21 st century. Achieving a sustainable balance is often a dilemma, especially in low-lying coastal areas where dams in inland river basin are trapping significant amounts of fluvial sediments. We recently investigated the shore of Bohai Bay in northern China where there has been a severe increase in sea level following a program of large-scale coastal reclamation and infrastructure development over the last five decades. To investigate this trend, we obtained sediment cores from near-shore in Bohai Bay, which were dated by ~(137)Cs and ~(210)Pb radionuclides to determine the sedimentation rates for the last 50 years. The average sedimentation rates of Bohai Bay exceeded 10 mm yr~(-1) before 1963, which was much higher than the rate of local sea-level rise. However, our results showed an overall decreasing sedimentation rate after 1963, which was not able to compensate for the increasing relative sea-level rise in that period. In addition, our results revealed that erosion occurred after the 1980 s in the shallow sea area of Bohai Bay. We suggest that this situation places the Bohai Bay coast at a greater risk of inundation and erosion within the next few decades than previously thought, especially in the large new reclamation area. This study may be a case study for many other shallow sea areas of the muddy coast if the sea level continues to rise rapidly and the sediment delivered by rivers continues to decrease. 相似文献